https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47691 Wed 25 Jan 2023 08:42:57 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13449 Wed 11 Apr 2018 17:21:20 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51638 Tue 12 Sep 2023 20:15:14 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33031 Tue 03 Sep 2019 17:55:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:552 Thu 25 Jul 2013 09:10:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4609 Sat 24 Mar 2018 10:13:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1717 Sat 24 Mar 2018 08:27:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18808 Sat 24 Mar 2018 07:51:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:193 Sat 24 Mar 2018 07:42:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:103 Sat 24 Mar 2018 07:42:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:250 Sat 24 Mar 2018 07:42:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26253 Sat 24 Mar 2018 07:40:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44457 p < 0.05, n = 5). Relative elongation of axial length and the vitreous chamber depth were also observed. Using pooled samples from all individuals (n = 10) to build a species-specific retinal ion library for SWATH analysis, 3202 non-redundant proteins (with 24,616 peptides) were identified at 1% global FDR. For quantitative analysis, the 10 individual retinal samples (5 pairs) were analyzed using a high resolution Triple-TOF 6600 mass spectrometry (MS) with technical replicates. In total, 37 up-regulated and 21 down-regulated proteins were found significantly changed after LIM treatment (log2 ratio (T/C) > 0.26 or < −0.26; p ≤ 0.05). Data are accepted via ProteomeXchange with identifier PXD025003. Through Ingenuity Pathways Analysis (IPA), “lipid metabolism” was found as the top function associated with the differentially expressed proteins. Based on the protein abundance and peptide sequences, expression patterns of two regulated proteins (SLC6A6 and PTGES2) identified in this pathway were further successfully validated with high confidence (p < 0.05) using a novel Multiple Reaction Monitoring (MRM) assay on a QTRAP 6500+ MS. In summary, through an integrated discovery and targeted proteomic approach, this study serves as the first report to detect and confirm novel retinal protein changes and significant biological functions in the early LIM mammalian guinea pigs. The study provides new workflow and insights for further research to myopia control.]]> Mon 24 Jul 2023 18:11:16 AEST ]]>